CPD course for Medical Laboratory Officers
Essentials of Polymerase Chain Reaction: Part 2 PCR Simulator: Practical 2026: (3 CPD)
3 CPD points · All levels · 5 lessons
About this course
By the end of the PCR simulator lesson, the learner should be able to:
Identify the main parts and settings of a PCR amplifier/thermal cycler.
Set basic PCR cycling conditions, including denaturation, annealing, extension, cycle number, and hold temperature.
Explain how temperature changes drive DNA denaturation, primer annealing, and strand extension.
Demonstrate how repeated PCR cycles lead to amplification of the target DNA sequence.
Interpret the PCR amplification graph produced by the simulator.
Predict how changes in PCR variables may affect amplification efficiency and final output.
Relate the simulator experience to the practical use of PCR in medical laboratory diagnosis and research.
Course curriculum
Foundations of PCR
Introduction
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How to use the PCR simulator
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PCR simulation
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PCR quiz
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Congratulations
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Your instructors
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